Anti-ARSE/ASE antibody (ab238485)
Key features and details
- Rabbit polyclonal to ARSE/ASE
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARSE/ASE antibody
See all ARSE/ASE primary antibodies -
Description
Rabbit polyclonal to ARSE/ASE -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ARSE/ASE aa 350-500.
Database link: P51690 -
Positive control
- IHC-P: Human liver and pancreatic tissues. ICC/IF: HepG2 cells.
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General notes
Previously labelled as ARSE
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ARSE/ASE antibody (ab238485)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ARSE/ASE (green) using ab238485 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
The cells were blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSE/ASE antibody (ab238485)Paraffin-embedded human liver tissue stained for ARSE/ASE using ab238485 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARSE/ASE antibody (ab238485)Paraffin-embedded human pancreatic tissue stained for ARSE/ASE using ab238485 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
