Recombinant human PIST + ROS1 protein (ab204105)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PIST + ROS1 protein -
Biological activity
The specific activity of ab204105 was determined to be 210 nmol/min/mg.
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Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MSAGGPCPAA AGGGPGGASC SVGAPGGVSM FRWLEVLEKE FDKAFVDVDL LLGEIDPDQA DITYEGRQKM TSLSSCFAQL CHKAQSVSQI NHKLEAQLVD LKSELTETQA EKVVLEKEVH DQLLQLHSIQ LQLHAKTGQS ADSGTIKAKL SGPSVEELER ELEANKKEKM KEAQLEAEVK LLRKENEALR RHIAVLQAEV YGARLAAKYL DKELAGRVQQ IQLLGRDMKG PAHDKLWNQL EAEIHLHRHK TVIRACRGRN DLKRPMQAPP GHDQDSLKKS QGVGPIRKVL LLKEDHEGLG ISITGGKEHG VPILISEIHP GQPADRCGGL HVGDAILAVN GVNLRDTKHK EAVTILSQQR GEIEFEVVYV APEVDSDDEN VEYEDESGHR YRLYLDELEG GGNPGASCKD TSGEIKVLQ -
Predicted molecular weight
145 kDa including tags -
Amino acids
1 to 419 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_020399.
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Amino Acid Sequence 2
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Species
Human -
Sequence
VWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPT QEEIENLPAFPR EKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVA VKTLKKGSTDQEKIEFLKEAHLMS KFNHPNILKQLGVCLLNEPQYIIL ELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVD ISKGCVYLERMH FIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGL LPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLN YVQTGGRLEP PRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRN FFLNSIYKSRDEANNSGVINES FEGEDGDVICLNSDDIMPVALMETKN REGLNYMVLATECGQGEEKSEGPLGSQESESCGL RKEEKEPHADKDFC QEKQVAYCPSGKPEGLNYACLTHSGYGDGSD -
Amino acids
1881 to 2347 -
Additional sequence information
NM_002944.
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Preparation and Storage
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Alternative names
- c-Ros receptor tyrosine kinase
- CAL
- CFTR-associated ligand
see all -
Relevance
PIST: Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. ROS1: Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2. -
Cellular localization
GOPC: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus; trans-Golgi network membrane; Peripheral membrane protein. Cell junction; synapse. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; dendrite ROS1: Cell membrane; Single-pass type I membrane protein.
Images
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Functional Studies - Recombinant human PIST + ROS1 protein (ab204105)
Kinase Assay demonstrating specific activity of ab204105.
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SDS-PAGE - Recombinant human PIST + ROS1 protein (ab204105)SDS-PAGE analysis of ab204105.