Anti-TNF alpha antibody (ab9739)
Key features and details
- Rabbit polyclonal to TNF alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-TNF alpha antibody
See all TNF alpha primary antibodies -
Description
Rabbit polyclonal to TNF alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Mouse TNF alpha aa 80-235. E.coli-derived recombinant MurineTNF-alpha
Database link: P06804 -
Positive control
- Recombinant mouse TNF alpha protein (ab9740) can be used as a positive control in WB.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1793.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-TNF alpha antibody (ab9739)
To detect mouse TNF alpha by Western Blot analysis, ab9739 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse TNF alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse TNF alpha, respectively.
Non-reducing conditions.
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Sandwich ELISA - Anti-TNF alpha antibody (ab9739)ELISA analysis of ab9739. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF alpha.
