Human TNFAIP3 knockout A549 cell lysate (ab257113)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262029 - Human TNFAIP3 knockout A549 cell lysate 1 x 100µg ab255554 - Human wild-type A549 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human TNFAIP3 knockout A549 cell lysate (ab257113)
Lane 1:Wild-type A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 2:TNFAIP3 knockout A549 (Human lung carcinoma cell line) whole cell lysate (20 ug)
Lane 3:Jurkat (Human T cell leukemia cell line from peripheral blood) cell treated with 5ng/ml PMA for 48 hours and then treated with 2ug/ml PHA for 48 hours, whole cell lysate (20 ug)
Lane 4:Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (20 ug)ab92324 was shown to specifically react with TNFAIP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266945 (knockout cell lysate ab257113) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TNFAIP3 knockout A549 cell lysate (ab257113)Homozygous: 10 bp deletion in exon7
