Human Mesothelin Matched Antibody Pair Kit (ab216051)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.62 pg/ml - 1000 pg/ml
Overview
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Product name
Human Mesothelin Matched Antibody Pair Kit
See all Mesothelin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
7.57 pg/ml -
Range
15.62 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Mesothelin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Mesothelin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216168. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Mesothelin Capture Antibody 2 x 50µg 1 x 50µg Human Mesothelin Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Mesothelin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Alternative names
- CAK 1
- CAK1
- CAK1 antigen
see all -
Database links
- Entrez Gene: 10232 Human
- Omim: 601051 Human
- SwissProt: Q13421 Human
- Unigene: 408488 Human
Images
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Human Mesothelin standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Mesothelin Matched Antibody Pair Kit (ab216051)To learn more about the advantages of recombinant antibodies see here.
