Human Mesothelin ELISA Kit (ab216168)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 132.5 pg/ml
- Range: 0.39 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Mesothelin ELISA Kit
See all Mesothelin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.7% Inter-assay Sample n Mean SD CV% Serum 3 3.3% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
132.5 pg/ml -
Range
0.39 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 92% - 97% Saliva 104 100% - 107% Urine 87 85% - 89% Serum 89 84% - 95% Hep Plasma 84 81% - 87% EDTA Plasma 98 88% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Mesothelin ELISA Kit (ab216168) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mesothelin protein in edta plasma, hep plasma, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Mesothelin with 132.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mesothelin is a 40 kDa GPI-anchored cell surface protein found on mesothelial cells and overexpressed in several types of cancer. The gene that encodes mesothelin is spliced into two different gene products, mesothelin and megakaryocyte potentiation factor. Mesothelin is involved in cellular adhesion, but is also secreted and normally found in several biological fluids.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Mesothelin Capture Antibody 1 x 600µl 10X Human Mesothelin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Mesothelin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Alternative names
- CAK 1
- CAK1
- CAK1 antigen
see all -
Database links
- Entrez Gene: 10232 Human
- Omim: 601051 Human
- SwissProt: Q13421 Human
- Unigene: 408488 Human
Images
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Other - Human Mesothelin ELISA Kit (ab216168)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Mesothelin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Mesothelin in human serum, plasma, urine and saliva samples.The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 37.5%, plasma (EDTA) 37.5%, urine 50% and saliva 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mesothelin concentration was determined to be 24.50 ng/mL in serum, 17.17 ng/mL in plasma (heparin), 24.54 ng/mL in plasma (EDTA), 12.37 ng/mL in urine and 277.2 ng/mL in saliva.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Mesothelin concentration was determined to be 25.79 ng/mL with a range of 6.86 – 60.93 ng/mL.
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Sandwich ELISA - Human Mesothelin ELISA Kit (ab216168)To learn more about the advantages of recombinant antibodies see here.
