Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11168] to Apc6 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Apc6 antibody [EPR11168] - BSA and Azide free
    See all Apc6 primary antibodies

  • Description

    Rabbit monoclonal [EPR11168] to Apc6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Apc6. The exact sequence is proprietary.
    Database link: Q13042

  • General notes

    Ab249488 is the carrier-free version of ab169536. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249488 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    All lanes : Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution

    Lane 1 : HT-1080 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 71 kDa



    This data was developed using ab169536, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 71 kDa



    This data was developed using ab169536, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    This data was developed using ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    This data was developed using ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    This data was developed using ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Apc6 with ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Immunocytochemistry - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    This data was developed using ab169536, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169536 at 1/100 dilution.
  • Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
    Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)

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