Human IKBKB (IKK beta) knockout HeLa cell lysate (ab257228)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260100 - Human IKBKB knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human IKBKB (IKK beta) knockout HeLa cell lysate (ab257228)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: IKBKB knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab124957 Anti-IKK beta antibody [EPR6043] was shown to specifically react with IKK beta in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264847 (knockout cell lysate ab257228) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124957 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human IKBKB (IKK beta) knockout HeLa cell lysate (ab257228)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: IKBKB knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32135 observed at 87 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32135 Anti-IKK beta antibody [Y466] was shown to specifically react with IKK beta in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264847 (knockout cell lysate ab257228) lane below 87kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32135 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human IKBKB knockout HeLa cell lysate (ab257228)Homozygous: 14 bp deletion in exon 6
