Recombinant human CD47 protein (Active) (ab271439)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human CD47 protein (Active)
See all CD47 proteins and peptides -
Biological activity
Assay Description: Coated wells in assay plate with either PBS (No Coat) or 250 ng/well of CD47-His in 50 μl PBS (overnight at 4 deg). Mixed various concentrations of SIRPα -biotin (0-3000nM) in assay buffer and added to assay plate wells. Incubated for 2h at RT. Detected binding with Strep-HRP generated luminescence. Assay Buffer is PBS + 0.1% BSA.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS P -
Predicted molecular weight
15 kDa including tags -
Molecular weight information
This protein runs at a higher molecular weight SDS-PAGE due to glycosylation. -
Amino acids
19 to 139 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human CD47 protein (Active) (ab271439)
SDS-PAGE analysis of 2 µg ab271439.
This protein runs at a higher molecular weight due to glycosylation.
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Functional Studies - Recombinant human CD47 protein (Active) (ab271439)CD47:SIRPα binding activity of ab271439.