Mouse galectin 9/Gal-9 ELISA Kit (ab277407)
Key features and details
- Sensitivity: 0.18 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Overview
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Product name
Mouse galectin 9/Gal-9 ELISA Kit
See all galectin 9/Gal-9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.18 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 81.08 71% - 95% Plasma 80.85 68% - 94% Cell culture media 80.49 70% - 92% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse galectin 9/Gal-9 ELISA kit (ab277407) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse galectin 9/Gal-9 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse galectin 9/Gal-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse galectin 9/Gal-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse galectin 9/Gal-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse galectin 9/Gal-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse galectin9/Gal-9 Antibody 2 vials Anti-Mouse galectin 9 Gal-9 coated Microplate 1 unit Mouse galectin 9/Gal-9 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. -
Tissue specificity
Peripheral blood leukocytes and lymphatic tissues. Overexpressed in Hodgkin disease tissue. -
Sequence similarities
Contains 2 galectin domains. -
Domain
Contains two homologous but distinct carbohydrate-binding domains. -
Cellular localization
Cytoplasm. Secreted. May also be secreted by a non-classical secretory pathway. - Information by UniProt
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Alternative names
- 36 kDa beta-galactoside-binding lectin
- Ecalectin
- Gal-9
see all -
Database links
- Entrez Gene: 16859 Mouse
- SwissProt: O08573 Mouse
- Unigene: 341434 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.