Recombinant mouse EPO protein (ab276979)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant mouse EPO protein
See all EPO proteins and peptides -
Biological activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is typically 2-10 ng/mL. -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK LYTGEVCRRG DR -
Predicted molecular weight
19 kDa including tags -
Molecular weight information
The recombinant mouse Epo consists 166 amino acids and predicts a molecular mass of 18.6 kDa. -
Amino acids
1 to 192 -
Additional sequence information
Predicted N-terminus: Ala 27.
Specifications
Our Abpromise guarantee covers the use of ab276979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- EP
- EPO
- EPO alpha
see all -
Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted
Images
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Functional Studies - (ab276979)
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is typically 2-10 ng/mL. -
SDS-PAGE - (ab276979)SDS-PAGE analysis of ab276979
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276979 has not yet been referenced specifically in any publications.
Preparation and Storage
- EP
- EPO
- EPO alpha
Images
-
Functional Studies - (ab276979)
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The ED50 for this effect is typically 2-10 ng/mL. -
SDS-PAGE - (ab276979)SDS-PAGE analysis of ab276979