Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant mouse EPO protein (ab276979)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant mouse EPO protein
    See all EPO proteins and peptides

  • Biological activity

    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
    The ED50 for this effect is typically 2-10 ng/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P07321

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      MGVPERPTLL LLLSLLLIPL GLPVLCAPPR LICDSRVLER YILEAKEAEN VTMGCAEGPR LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK LYTGEVCRRG DR

    • Predicted molecular weight

      19 kDa including tags

    • Molecular weight information

      The recombinant mouse Epo consists 166 amino acids and predicts a molecular mass of 18.6 kDa.

    • Amino acids

      1 to 192

    • Additional sequence information

      Predicted N-terminus: Ala 27.

    • Specifications

    Our Abpromise guarantee covers the use of ab276979 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • EP
      • EPO
      • EPO alpha
      • Epoetin
      • Erythropoetin
      • Erythropoietin precursor
      • MVCD2
      see all

    • Relevance

      Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

    • Cellular localization

      Secreted

Images

  • Functional Studies - (ab276979)
    Functional Studies - (ab276979)

    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
    The ED50 for this effect is typically 2-10 ng/mL.

  • SDS-PAGE - (ab276979)
    SDS-PAGE - (ab276979)
    SDS-PAGE analysis of ab276979

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab276979? Please let us know so that we can cite the reference in this datasheet.

ab276979 has not yet been referenced specifically in any publications.

Preparation and Storage

  • EP
  • EPO
  • EPO alpha

Images

  • Functional Studies - (ab276979)
    Functional Studies - (ab276979)

    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
    The ED50 for this effect is typically 2-10 ng/mL.

  • SDS-PAGE - (ab276979)
    SDS-PAGE - (ab276979)
    SDS-PAGE analysis of ab276979

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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