Anti-Angiotensin II Type 1 Receptor antibody (ab59018)
Key features and details
- Goat polyclonal to Angiotensin II Type 1 Receptor
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Angiotensin II Type 1 Receptor antibody
See all Angiotensin II Type 1 Receptor primary antibodies -
Description
Goat polyclonal to Angiotensin II Type 1 Receptor -
Host species
Goat -
Specificity
All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).
Preliminary testing was unsuccessful on Rat Liver in Western blot.
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Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide:
C-EIQKNKPRNDDIFK
, corresponding to internal sequence amino acids 227-240 of Human Angiotensin II Type 1 Receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Angiotensin II Type 1 Receptor antibody (ab59018)All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab59018) at 0.2 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Human liver lysate with Immunizing peptide
Lane 3 : Mouse liver lysate
Lane 4 : Mouse liver lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 41 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 45, 75 kDa. We are unsure as to the identity of these extra bands.Primary incubation: 1 hour at room temperature.
Detected by chemiluminescence.
