Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free
    See all Angiotensin II Type 1 Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).

  • General notes

    Ab239995 is the carrier-free version of ab124734. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa



    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 41 kDa



    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa



    This data was developed using ab124734, the same antibody clone in a different buffer formulation.

     

  • Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free (ab239995)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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