Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
    See all Angiotensin II Type 1 Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ELISA
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P30556

  • Positive control

    • WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

    ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa

  • Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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