Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

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Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
Suitable for: WB, ELISA
Reacts with: Mouse, Rat, Human

Product name

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
See all Angiotensin II Type 1 Receptor primary antibodies
Description

Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
Host species

Rabbit
Specificity

The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
ELISA	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P30556
Positive control
- WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3873
Isotype

IgG
Research areas

Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)

Lane 1 : C2C12 cell lysate
Lane 2 : PC-12 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 41 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 41 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)

Lane 1 : C2C12 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 41 kDa
Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

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