Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
![Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-107896-anti-ampk-alpha-1-phospho-s496-antibody-epr3051-western-blot.jpg "Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051]
See all AMPK alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR3051] to AMPK alpha 1 (phospho S496) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
A phospho-specific peptide corresponding to residues surrounding Serine 487 of Human AMPK alpha 1.
-
Positive control
- 293T cell lysate; HeLa cells
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3051 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution
Lane 1 : 293T cell lysate untreated
Lane 2 : 293T cell lysate treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
-
![Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-107895-anti-ampk-alpha-1-phospho-s496-antibody-epr3051-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution. -
![Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)](https://www.abcam.com/ps/products/92/ab92701/Images/ab92701-3-benefits-of-recombinant-antibodies.png)
Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)
