Anti-AMPK alpha 1 antibody [2B7] (ab110036)
![Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123077-anti-ampk-alpha-1-antibody-2b7-western-blot.jpg "Anti-AMPK alpha 1 antibody [2B7] (ab110036)")
Key features and details
- Mouse monoclonal [2B7] to AMPK alpha 1
- Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-AMPK alpha 1 antibody [2B7]
See all AMPK alpha 1 primary antibodies -
Description
Mouse monoclonal [2B7] to AMPK alpha 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt RatICC/IF HumanWB Human
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Immunogen
Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.
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Positive control
- Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2B7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : HeLa cell lysate.
Lane 3 : HepG2 cell lysate.
Lane 4 : MCF-7 cell lysate.
Lane 5 : Cos7 cell lysate.
Lane 6 : NIH3T3 cell lysate.
Lane 7 : K562 cell lysate.
Lane 8 : HEK293 cell lysate.
Lane 9 : PC-12 cell lysate.
Predicted band size: 64 kDa
Observed band size: 64 kDa
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![ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123076-anti-ampk-alpha-1-antibody-2b7-elisa.jpg)
ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)The proposed dilution of ab110036 is 1/10000. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123075-anti-ampk-alpha-1-antibody-2b7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123074-anti-ampk-alpha-1-antibody-2b7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). -
![Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123073-anti-ampk-alpha-1-antibody-2b7-flow-cytometry.jpg)
Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)](https://www.abcam.com/ps/products/110/ab110036/Images/ab110036-123072-anti-ampk-alpha-1-antibody-2b7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.
