Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)
![Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)](https://www.abcam.com/ps/products/251/ab251206/Images/ab251206-405734-anti-alkbh4-antibody-epr19216-bsa-and-azide-free-western-blot.jpg "Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19216] to ALKBH4 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free
See all ALKBH4 primary antibodies -
Description
Rabbit monoclonal [EPR19216] to ALKBH4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251206 is the carrier-free version of ab195379. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251206 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19216 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1 minuteThis data was developed using ab195379, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)](https://www.abcam.com/ps/products/251/ab251206/Images/ab251206-403455-anti-alkbh4-antibody-epr19216-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)All lanes : Anti-ALKBH4 antibody [EPR19216] (ab195379) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 4 : Human pancreas tissue lysate
Lane 5 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 1 minuteThis data was developed using ab195379, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)](https://www.abcam.com/ps/products/251/ab251206/Images/ab251206-1-anti-alkbh4-antibody-epr19216-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)This data was developed using ab195379, the same antibody clone in a different buffer formulation.ALKBH4 was immunoprecipitated from 0.35 mg of HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with ab195379 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab195379 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input).
Lane 2: ab195379 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195379 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds. -
![Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)](https://www.abcam.com/ps/products/251/ab251206/Images/ab251206-4-benefits-of-recombinant-antibodies.png)
Anti-ALKBH4 antibody [EPR19216] - BSA and Azide free (ab251206)