Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24317-33] to MARCO - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MARCO antibody [EPR24317-33] - BSA and Azide free
    See all MARCO primary antibodies

  • Description

    Rabbit monoclonal [EPR24317-33] to MARCO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: LPS treated J774A.1 cells. Flow Cyt: LPS treated J774A.1 cells. Mouse bone marrow treated with GM-CSF and IL-4.

  • General notes

    ab278507 is the carrier-free version of ab271060. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278507 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24317-33

  • Isotype

    IgG

Images

  • Immunocytochemistry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)
    Immunocytochemistry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)

    This data was developed using ab271060, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized J774A.1 cells labelling MARCO with ab271060 at 1/500 (0.982 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing membranous and cytoplasmic staining in J774A.1 cell line treated with lipopolysaccharide (1 µg/ml) for 24 hours.

    ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)
    Flow Cytometry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)

    This data was developed using ab271060, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) treated with 500 ng/ml lipopolysaccharide (LPS) for 3 days (Red)/ Untreated control (Green) cells labelling MARCO with ab271060 at 1/500 dilution (0.1 µg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)
    Flow Cytometry - Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)

    This data was developed using ab271060, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of mouse bone marrow cells treated with 10 ng/mL GM-CSF+5 ng/mL IL-4 for 24h (Right) / Untreated control (Left) cells labelling MARCO with ab271060 at 1/50 dilution (1 µg). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab271060, then stained with anti-CD11c conjugated to Alexa Fluor® 647 and MHC class II (I-A/I-E) conjugated to SB702.

    Gated on viable CD11c positive population.

  • Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)
    Anti-MARCO antibody [EPR24317-33] - BSA and Azide free (ab278507)

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