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Human Sortilin/NT3 ELISA Kit (ab277461)

Key features and details

  • Sensitivity: 0.81 ng/ml
  • Range: 0.819 ng/ml - 200 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Sortilin/NT3 ELISA Kit
    See all Sortilin/NT3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.81 ng/ml

  • Range

    0.819 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 91.44 80% - 111%
    Plasma 90.11 82% - 107%
    Cell culture media 103.3 97% - 119%

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Sortilin/NT3 ELISA Kit (ab277461) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Sortilin/NT3 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Sortilin/NT3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Sortilin/NT3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Sortilin/NT3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Sortilin/NT3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated Anti-Human Sortilin/NT3 Antibody 2 vials
    Anti-Human Sortilin NT3 coated Microplate 1 unit
    Human Sortilin/NT3 standard protein (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

  • Tissue specificity

    Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.

  • Involvement in disease

    Note=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.

  • Sequence similarities

    Belongs to the VPS10-related sortilin family. SORT1 subfamily.
    Contains 9 BNR repeats.

  • Domain

    The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
    The extracellular domain may be shed following protease cleavage in some cell types.

  • Post-translational
    modifications

    The N-terminal propeptide is cleaved by furin and possibly other homologous proteases.

  • Cellular localization

    Membrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
  • Information by UniProt

  • Alternative names

    • 100 kDa NT receptor
    • Glycoprotein 95
    • Gp 95
    • Gp95
    • LDLCQ6
    • Neurotensin receptor 3
    • NT 3
    • NT3
    • NTR 3
    • NTR3
    • OTTHUMP00000013784
    • SORT 1
    • SORT_HUMAN
    • Sort1
    • SORT1 (gene name)
    • Sortilin
    • Sortilin 1
    see all

  • Database links

    Images

    • Example data
      Example data

      These standard curves are for demonstration only. A standard curve must be run with each assay.

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