Anti-ALK (phospho Y1507) antibody (ab73996)
Key features and details
- Rabbit polyclonal to ALK (phospho Y1507)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-ALK (phospho Y1507) antibody
See all ALK primary antibodies -
Description
Rabbit polyclonal to ALK (phospho Y1507) -
Host species
Rabbit -
Specificity
ab73996 detects ALK only when phosphorylated at tyrosine 1507. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey -
Immunogen
Synthetic phospho-peptide derived from human ALK around the phosphorylation site of tyrosine 1507 (P-T-YP-G-S).
-
Positive control
- human brain tissue, extracts from COS cells, treated with anisomycin (25ug/ml, 30mins)
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-ALK (phospho Y1507) antibody (ab73996) at 1/500 dilution
Lane 1 : extracts from COS cells, treated
with anisomycin (25ug/ml, 30mins)
Lane 2 : extracts from COS cells, treated
with anisomycin (25ug/ml, 30mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 176 kDa
Observed band size: 176 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK (phospho Y1507) antibody (ab73996)Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab73996. Left: untreated sample. Right: sample treated with immunising peptide.