Anti-AK5 antibody [OTI1D6] (ab117927)
![Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.com/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-1.jpg "Anti-AK5 antibody [OTI1D6] (ab117927)")
Key features and details
- Mouse monoclonal [OTI1D6] to AK5
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-AK5 antibody [OTI1D6]
See all AK5 primary antibodies -
Description
Mouse monoclonal [OTI1D6] to AK5 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human AK5 aa 1-600. NP_777283. Produced in HEK-293T cells.
Database link: Q9Y6K8 -
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA. ICC/IF: COS-7 transiently transfected by pCMV6-ENTRY AK5 cDNA. Flow Cyt: HEK-293T cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1.
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General notes
Clone OTI1D6 (formerly 1D6).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1D6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)All lanes : Anti-AK5 antibody [OTI1D6] (ab117927) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.com/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)pCMV6-ENTRY AK5 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for AK5 using ab117927 at a 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.com/ps/products/117/ab117927/Images/ab117927-314116-117927777.jpg)
Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)Flow Cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1, using ab117927 (red) compared to an empty vector control plasmid (blue).
