Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13120] to AGPS - N-terminal
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-AGPS antibody [EPR13120] - N-terminal
    See all AGPS primary antibodies

  • Description

    Rabbit monoclonal [EPR13120] to AGPS - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, SW480, K562, HepG2 whole cell lysate (ab7900); Human cervical carcinoma tissue; HeLa cells

  • General notes

    This product was previously labelled as Alkyl-DHAP synthase.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13120

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Flow cytometric analysis of  2% paraformaldehyde fixed HeLa cells labeling AGPS with ab184186 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

  • Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling AGPS with ab184186 at a 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 diluiton.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling AGPS with ab184186 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)
    Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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