Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

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Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13120] to AGPS - N-terminal
Suitable for: WB, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-AGPS antibody [EPR13120] - N-terminal
See all AGPS primary antibodies
Description

Rabbit monoclonal [EPR13120] to AGPS - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, SW480, K562, HepG2 whole cell lysate (ab7900); Human cervical carcinoma tissue; HeLa cells
General notes
This product was previously labelled as Alkyl-DHAP synthase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13120
Isotype

IgG
Research areas

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

All lanes : Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/2000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 73 kDa
Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling AGPS with ab184186 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
Flow Cytometry - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling AGPS with ab184186 at a 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 diluiton.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling AGPS with ab184186 at a 1/100 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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