Anti-ABL1 antibody (ab272881)
Key features and details
- Rabbit polyclonal to ABL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ABL1 antibody
See all ABL1 primary antibodies -
Description
Rabbit polyclonal to ABL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ABL1 (C terminal). The exact sequence is proprietary.
Database link: P00519 -
Positive control
- WB: NT2/D1 and K562 whole cell lysate. IHC-P: Human hepatoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ABL1 antibody (ab272881)Anti-ABL1 antibody (ab272881) at 1/1000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABL1 antibody (ab272881)Paraffin-embedded human hepatoma tissue stained for ABL1 using ab272881 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-ABL1 antibody (ab272881)4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ABL1 using ab272881 at 1/100 dilution in ICC/IF. Blue: Hoechst 33342 staining.
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Western blot - Anti-ABL1 antibody (ab272881)All lanes : Anti-ABL1 antibody (ab272881) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody