Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µg 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-6X His tag® antibody [RM146] (ab200537)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM146] to 6X His tag®
Suitable for: ICC, Flow Cyt, WB, IP
Reacts with: Species independent

Product name

Anti-6X His tag® antibody [RM146]
See all 6X His tag® primary antibodies
Description

Rabbit monoclonal [RM146] to 6X His tag®
Host species

Rabbit
Specificity

ab200537 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Tested applications

Suitable for: ICC, Flow Cyt, WB, IPmore details
Species reactivity

Reacts with: Species independent
Immunogen

Synthetic peptide corresponding to 6X His tag®. Mixture of a peptide with 6X His tag® at the N-terminus and another peptide with 6X His tag® at the C-terminus.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from an animal origin–free culture supernatant.
Clonality

Monoclonal
Clone number

RM146
Isotype

IgG
Research areas

Western blot - Anti-6X His tag® antibody [RM146] (ab200537)

All lanes : Anti-6X His tag® antibody [RM146] (ab200537) at 0.2 µg/ml

Lane 1 : Untransfected 293T cells
Lane 2 : Transfected with His-Tag fusion protein X 293T cells
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y

The diluent was 5% milk/TBST.
Immunoprecipitation - Anti-6X His tag® antibody [RM146] (ab200537)

ab200537 at 0.5 - 2 µg/mL immunoprecipitating 6X His tag® in 293T whole cell lysate.

Lane 1 (input): 293T whole lysate control

Lane 2 (+): Rabbit monoclonal IgG instead of ab200537 in 293T whole cell lysate

Lane 3 (-): ab200537 + 293T whole cell lysate
Immunocytochemistry - Anti-6X His tag® antibody [RM146] (ab200537)

Immunohistochemical analysis of 293T cells expressing His-Tag nuclear protein X with ab200537 at 0.1 - 1 µg/mL. The negative control in the inset shows immunohistochemical staining of untransfected HepG2 cells with ab200537 at 0.1 - 1 µg/mL.
Anti-6X His tag® antibody [RM146] (ab200537)

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