Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-6X His tag® antibody [RM146] (ab200537)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM146] to 6X His tag®
  • Suitable for: ICC, Flow Cyt, WB, IP
  • Reacts with: Species independent

Overview

  • Product name

    Anti-6X His tag® antibody [RM146]
    See all 6X His tag® primary antibodies

  • Description

    Rabbit monoclonal [RM146] to 6X His tag®

  • Host species

    Rabbit

  • Specificity

    ab200537 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

  • Tested applications

    Suitable for: ICC, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to 6X His tag®. Mixture of a peptide with 6X His tag® at the N-terminus and another peptide with 6X His tag® at the C-terminus.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM146

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-6X His tag® antibody [RM146] (ab200537)
    Western blot - Anti-6X His tag® antibody [RM146] (ab200537)
    All lanes : Anti-6X His tag® antibody [RM146] (ab200537) at 0.2 µg/ml

    Lane 1 : Untransfected 293T cells
    Lane 2 : Transfected with His-Tag fusion protein X 293T cells
    Lane 3 : E. coli lysate without His-Tag Protein Y
    Lane 4 : E. coli lysate with His-Tag Protein Y


    The diluent was 5% milk/TBST.

  • Immunoprecipitation - Anti-6X His tag® antibody [RM146] (ab200537)
    Immunoprecipitation - Anti-6X His tag® antibody [RM146] (ab200537)

    ab200537 at 0.5 - 2 µg/mL immunoprecipitating 6X His tag® in 293T whole cell lysate.

    Lane 1 (input): 293T whole lysate control

    Lane 2 (+): Rabbit monoclonal IgG instead of ab200537 in 293T whole cell lysate

    Lane 3 (-): ab200537 + 293T whole cell lysate

  • Immunocytochemistry - Anti-6X His tag® antibody [RM146] (ab200537)
    Immunocytochemistry - Anti-6X His tag® antibody [RM146] (ab200537)

    Immunohistochemical analysis of 293T cells expressing His-Tag nuclear protein X with ab200537 at 0.1 - 1 µg/mL. The negative control in the inset shows immunohistochemical staining of untransfected HepG2 cells with ab200537 at 0.1 - 1 µg/mL. 

  • Anti-6X His tag® antibody [RM146] (ab200537)
    Anti-6X His tag® antibody [RM146] (ab200537)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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