Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free
    See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251232 is the carrier-free version of ab197025 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251232 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    This data was developed using ab197025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    This data was developed using ab197025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    This data was developed using ab197025, the same antibody clone in a different buffer formulation.2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
    Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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