Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)
![Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)](https://www.abcam.com/ps/products/251/ab251232/Images/ab251232-408119-anti-2-hydroxy-phytanoyl-coa-lyase-hacl1-antibody-epr16812-bsa-and-azide-free-western-blot.jpg "Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free
See all 2 Hydroxy phytanoyl CoA lyase/HACL1 primary antibodies -
Description
Rabbit monoclonal [EPR16812] to 2 Hydroxy phytanoyl CoA lyase/HACL1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251232 is the carrier-free version of ab197025 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251232 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as 2 Hydroxy phytanoyl CoA lyase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16812 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab197025, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)](https://www.abcam.com/ps/products/251/ab251232/Images/ab251232-405755-anti-2-hydroxy-phytanoyl-coa-lyase-hacl1-antibody-epr16812-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)All lanes : Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] (ab197025) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab197025, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)](https://www.abcam.com/ps/products/251/ab251232/Images/ab251232-1-anti-2-hydroxy-phytanoyl-coa-lyase-hacl1-antibody-epr16812-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)This data was developed using ab197025, the same antibody clone in a different buffer formulation.2 Hydroxy phytanoyl CoA lyase/HACL1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
![Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)](https://www.abcam.com/ps/products/251/ab251232/Images/ab251232-4-benefits-of-recombinant-antibodies.png)
Anti-2 Hydroxy phytanoyl CoA lyase/HACL1 antibody [EPR16812] - BSA and Azide free (ab251232)