Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24102-105] to Dopamine Receptor D1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free
    See all Dopamine Receptor D1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24102-105] to Dopamine Receptor D1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse striatum; Rat striatum. IHC-P: Mouse brain and corpus striatum; Rat corpus striatum.

  • General notes

    ab279718 is the carrier-free version of ab279713. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab279718 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)
    Western blot - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)
    All lanes : Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713) at 1/1000 dilution

    Lane 1 : Mouse striatum tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Rat striatum tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 49 kDa
    Observed band size: 95-100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab279713, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:  Lane 1-3: 15 seconds Lane 4-6: 69 seconds

    The molecular weight observed is consistent with what have been described in literature (PMID: 28316027).

    Negative control: heart, kidney (PMID: 2144334)

    Samples are non-boiled as boiling may cause protein aggregates.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)

    This data was developed using ab279713, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining is mainly on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)

    This data was developed using ab279713, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Mouse corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Receptor D1 antibody [EPR24102-105] - BSA and Azide free (ab279718)

    This data was developed using ab279713, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded Rat corpus striatum tissue labeling Dopamine Receptor D1 with ab279713 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat corpus striatum of mouse brain. The section was incubated with ab279713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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