Anti-14-3-3 tau antibody (ab264319)
Key features and details
- Rabbit polyclonal to 14-3-3 tau
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 tau antibody
See all 14-3-3 tau primary antibodies -
Description
Rabbit polyclonal to 14-3-3 tau -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Xenopus laevis, Orangutan
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Immunogen
Synthetic peptide aa 195-245. The exact sequence is proprietary. NP_006817.1
Database link: P27348 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HeLa, HEK-239T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-14-3-3 tau antibody (ab264319)All lanes : Anti-14-3-3 tau antibody (ab264319) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-14-3-3 tau antibody (ab264319)14-3-3 tau was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264319 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264319 at 1 µg/ml.
Lane 1: ab264319 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 tau antibody (ab264319)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling 14-3-3 tau with ab264319 at 1/5,000 dilution. Detection: DAB.
