Anti-VDAC1/Porin antibody (ab235143)
Key features and details
- Goat polyclonal to VDAC1/Porin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies -
Description
Goat polyclonal to VDAC1/Porin -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant human VDAC1 protein; Mouse heart lysate; HeLa cell lysate; Rat brain lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescent analysis of 100% methanol fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling VDAC1/Porin with ab235143 at 5 µg/ml (overnight at 4°C), followed by an anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
The nuclear counterstain is DAPI (blue).Panel A: DAPI.
Panel B: ab235143 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary antibody only. -
All lanes : Anti-VDAC1/Porin antibody (ab235143) at 1/1000 dilution
Lane 1 : PreStained Molecular weight
Lane 2 : full length recombinant human VDAC1
Lane 3 : HeLa Whole Cell Lysate
Lane 4 : Rat Brain Lysate
Secondary
All lanes : F(ab)'2 Donkey anti-Goat IgG (H&L)-HRP at 1/70000 dilution
Predicted band size: 31 kDa
Exposure time: 60 secondsDilution buffer: SDS.
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All lanes : Anti-VDAC1/Porin antibody (ab235143) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse heart lysate
Secondary
All lanes : F(ab)'2 Donkey anti-Goat IgG (H&L) - HRP at 1/70000 dilution
Predicted band size: 31 kDa
Exposure time: 60 secondsBlocking/Dilution buffer: Casein.