Anti-VDAC1/Porin antibody (ab223570)
Key features and details
- Rabbit polyclonal to VDAC1/Porin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies -
Description
Rabbit polyclonal to VDAC1/Porin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human VDAC1/Porin (N terminal).
Database link: P21796 -
Positive control
- WB: HeLa whole cell lysate. ICC/IF: RKO cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VDAC1/Porin antibody (ab223570)Anti-VDAC1/Porin antibody (ab223570) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 6 minutesBlocking buffer: 5% skim milk in TBST.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody (ab223570)4% formaldehyde-fixed RKO cells stained for VDAC1/Porin (green) using ab223570 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223570 (D) Merge.
