Anti-VDAC1/Porin antibody [N152B/23] (ab186321)
![Anti-VDAC1/Porin antibody [N152B/23] (ab186321)](https://www.abcam.com/ps/products/186/ab186321/Images/ab186321-312533-anti-vdac1-porin-antibody-n152b-23-immunofluorescence.jpg "Anti-VDAC1/Porin antibody [N152B/23] (ab186321)")
Key features and details
- Mouse monoclonal [N152B/23] to VDAC1/Porin
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-VDAC1/Porin antibody [N152B/23]
See all VDAC1/Porin primary antibodies -
Description
Mouse monoclonal [N152B/23] to VDAC1/Porin -
Host species
Mouse -
Specificity
ab186321 does not cross-react with VDAC2 or VDAC3 (based on KO validation results). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human VDAC1/Porin aa 1 to the C-terminus.
Database link: P21796 -
Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S152B-23 to N152B/23, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N152B/23 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] (ab186321)
4% formaldehyde-fixed SK-N-BE cells stained for VDAC1/Porin (green) using ab186321 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 minutes room temperature, 5 minutes room temperature. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab186321 (D) Composite.
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![Western blot - Anti-VDAC1/Porin antibody [N152B/23] (ab186321)](https://www.abcam.com/ps/products/186/ab186321/Images/ab186321-312531-anti-vdac1-porin-antibody-n152b-23-western-blot.jpg)
Western blot - Anti-VDAC1/Porin antibody [N152B/23] (ab186321)Anti-VDAC1/Porin antibody [N152B/23] (ab186321) at 1/200 dilution + Rat brain memebrane lysate at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Predicted band size: 31 kDa
