Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22865-73] to CD10 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD10 antibody [EPR22865-73] - BSA and Azide free
    See all CD10 primary antibodies

  • Description

    Rabbit monoclonal [EPR22865-73] to CD10 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC application is recommended for human only.

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta tisssue lysate; rat kidney and liver lysates; Raji, Ramos and HAP1 whole cell lysates; rat kidney and liver lysates; mouse kidney lysate. IHC-P: Human kidney, breast, liver and tonsil tissue. ICC/IF: Raji and Ramos cells. IP: Raji whole cell lysate.

  • General notes

    ab256767 is the carrier-free version of ab255609.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunoprecipitation - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    CD10 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab255609 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab255609 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Raji whole cell lysate 10 μg (Input).
    Lane 2: ab255609 IP in Raji whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255609 in Raji whole cell lysate.

     

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunofluorescent analysis of 100% methanol-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling CD10 with ab255609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Ramos cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).


    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.


    100% methanol was preferred as fixative.




    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).
  • Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling CD10 with ab255609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).


    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.


    100% methanol was preferred as fixative.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on germinal center of human tonsil (PMID:10843287) is observed. Counter stained with hematoxylin.

     

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on intrahepatic canaliculi of human liver (PMID:10705818) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on myoepithelial cells of human breast (PMID:10705818, 17143263) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on proximal convoluted tubules and glomerular epithelial cells of human kidney (PMID:10705818) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255609).

  • Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)
    Anti-CD10 antibody [EPR22865-73] - BSA and Azide free (ab256767)

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