Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Anti-CD10 antibody [SP179] - N-terminal (ab227659) at 1/400 dilution + LNCaP (human prostate cancer cell line) cell lysate

Predicted band size: 85 kDa

Flow cytometry analysis of 2.4G2 (Rat B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Flow cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte ) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/20 dilution (7.05 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Formalin-fixed, paraffin-embedded human kidney tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD10 with ab227659 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue). 

Formalin-fixed, paraffin-embedded human breast tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human placenta tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human liver tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.