Antibodies, Proteins, Kits and Reagents for Life Science

241 228 ₸ Product size

100 µl 241 228 ₸

Order now and get it on Friday March 12, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4378] to GRB7
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-GRB7 antibody [EPR4378]
See all GRB7 primary antibodies
Description

Rabbit monoclonal [EPR4378] to GRB7
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4378
Isotype

IgG
Research areas

Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)

All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)

Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)

ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)

All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

Lane 1 : SKBR-3 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 60 kDa
Anti-GRB7 antibody [EPR4378] (ab109618)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com