Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRB7 antibody [EPR4378] (ab109618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4378] to GRB7
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GRB7 antibody [EPR4378]
    See all GRB7 primary antibodies

  • Description

    Rabbit monoclonal [EPR4378] to GRB7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4378

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
    Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
    All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)

    Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB7 antibody [EPR4378] (ab109618)

    ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
    Western blot - Anti-GRB7 antibody [EPR4378] (ab109618)
    All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution

    Lane 1 : SKBR-3 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 60 kDa

  • Anti-GRB7 antibody [EPR4378] (ab109618)
    Anti-GRB7 antibody [EPR4378] (ab109618)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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