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Anti-GRB7 antibody [EPR6858] (ab129091)

Price and availability

247 929 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-GRB7 antibody [EPR6858] (ab129091)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6858] to GRB7
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-GRB7 antibody [EPR6858]
    See all GRB7 primary antibodies
  • Description

    Rabbit monoclonal [EPR6858] to GRB7
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues in Human GRB7 (Q14451).

  • Positive control

    • SKBR-3 whole cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6858
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic

Images

  • Western blot - Anti-GRB7 antibody [EPR6858] (ab129091)
    Western blot - Anti-GRB7 antibody [EPR6858] (ab129091)
    All lanes : Anti-GRB7 antibody [EPR6858] (ab129091) at 1/1000 dilution

    Lanes 1 & 5 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
    Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Exposure time: Lane 1-4: 180 seconds; Lane 5: 7 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The GRB7 expression level in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID 17916906 and 7907978.

    This image is derived from purified batch.

  • Anti-GRB7 antibody [EPR6858] (ab129091)
    Anti-GRB7 antibody [EPR6858] (ab129091)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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