Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (ab120488)
Key features and details
- Cell permeable caspase-3 inhibitor
- CAS Number: 210344-95-9
- Purity: > 90%
- Soluble in DMSO to 20 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor -
Description
Cell permeable caspase-3 inhibitor -
Alternative names
- Caspase-3 Inhibitor
- Z-DEVD-FMK (cell permeable)
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Biological description
A potent, cell-permeable, and irreversible inhibitor of caspase-3. Also inhibits caspase-6, caspase-7, caspase-8, and caspase-10. Once in the cell, endogenous esterase activity hydrolyzes the methyl groups to form the biologically active form. Therefore, when using with isolated, purified or recombinant caspase enzymes, pre-treatment with an esterase is required.
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Purity
> 90% -
CAS Number
210344-95-9 -
Chemical structure
Properties
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Molecular weight
668.67 -
Molecular formula
C30H41FN4O12 -
Sequence
DEVD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-1 = Asp(OMe); Glu-2 = Glu(OMe); Asp-4 = Asp(OMe)) -
PubChem identifier
16760394 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 20 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
FCC(=O)[C@H](CC(=O)OC)NC(=O)[C@@H](NC(=O)[C@H](CCC(=O)OC)NC(=O)[C@H](CC(=O)OC)NC(=O)OCc1ccccc1)C(C)C -
Source
Synthetic
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Research areas
Images
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Functional Studies - Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (ab120488)
HeLa cells were incubated at 37 °C for 1h with vehicle control (0 µM) and different concentrations of Z-D(OMe)E(OMe)VD(OMe)-FMK (ab120488). After this incubation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-D(OMe)E(OMe)VD(OMe)-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051
