Blocking - Blocking peptide for Agarose Anti-V5 tag antibody (ab1229), FITC Anti-V5 tag antibody (ab1274), HRP Anti-V5 tag antibody (ab1325), Anti-V5 tag antibody (ab9113), Anti-V5 tag antibody (ab9116), Anti-V5 tag antibody (ab9137), Anti-V5 tag antibody (ab15828)

- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.