Anti-V5 tag antibody (ab9116)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: WB, ELISA, IP, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Specificity
ELISA: V5 tag antibody - ChIP Grade (ab9116) specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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Tested applications
Suitable for: WB, ELISA, IP, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg . -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-V5 tag antibody (ab9116) This image is courtesy of an anonymous AbreviewAll lanes : Anti-V5 tag antibody (ab9116) at 1/5000 dilution
Lanes 1-3 : Cells transfected with v5-tagged protein.
Lanes 4-5 : Untransfected cells.
Secondary
Lanes 1-3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4-5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate.
