Anti-V5 tag antibody [SV5-Pk1] (ab27671)
![Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-380968-anti-v5-tag-antibody-western-blot-nih3t3-cell-lysate.jpg "Anti-V5 tag antibody [SV5-Pk1] (ab27671)")
Key features and details
- Mouse monoclonal [SV5-Pk1] to V5 tag
- Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIA
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-V5 tag antibody [SV5-Pk1]
See all V5 tag primary antibodies -
Description
Mouse monoclonal [SV5-Pk1] to V5 tag -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
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Epitope
ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. -
Positive control
- WB: Transfected NIH/3T3 and COS-7 cell lysates.
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General notes
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography. -
Primary antibody notes
Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope. -
Clonality
Monoclonal -
Clone number
SV5-Pk1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)All lanes : Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1-2 : Transfected NIH/3T3 cell lysate
Lane 3 : Not transfected NIH/3T3 cell lysate
Secondary
All lanes : HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1 secondCells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
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![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-318649-anti-v5-tag-antibody-sv5-pk1-western-blot.jpg)
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671) Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
