Anti-V5 tag antibody (ab15828)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: ChIP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Rabbit polyclonal to V5 tag -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab15828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ChIP Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. WB Use a concentration of 0.5 µg/ml. Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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ChIP - Anti-V5 tag antibody (ab15828)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
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Western blot - Anti-V5 tag antibody (ab15828)All lanes : Anti-V5 tag antibody (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho withV5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Datasheets and documents
References (33)
ab15828 has been referenced in 33 publications.
- Safiulina D et al. Miro proteins prime mitochondria for Parkin translocation and mitophagy. EMBO J 38:N/A (2019). PubMed: 30504269
- Federico A et al. The complex CBX7-PRMT1 has a critical role in regulating E-cadherin gene expression and cell migration. Biochim Biophys Acta Gene Regul Mech 1862:509-521 (2019). PubMed: 30826432
- Chen JY et al. R-ChIP for genome-wide mapping of R-loops by using catalytically inactive RNASEH1. Nat Protoc 14:1661-1685 (2019). PubMed: 30996261
- Kung-Chun Chiu D et al. Hypoxia regulates the mitochondrial activity of hepatocellular carcinoma cells through HIF/HEY1/PINK1 pathway. Cell Death Dis 10:934 (2019). PubMed: 31819034
- Dewari PS et al. An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein. Elife 7:N/A (2018). PubMed: 29638216
Images
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ChIP - Anti-V5 tag antibody (ab15828)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
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Western blot - Anti-V5 tag antibody (ab15828)All lanes : Anti-V5 tag antibody (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho withV5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
