E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
Overview
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Product name
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate -
General notes
This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
GST
T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Lysate notes
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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Research areas
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Background
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Images
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Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution
Lane 1 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
Lane 3 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
Lane 4 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl -
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
