Resorufin ß-D-galactopyranoside (ab273639)
Key features and details
- Red fluorogenic substrate for lacZ ß-galactosidase activity, intracellularly, or in vitro.
- CAS Number: 95079-19-9
Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Resorufin ß-D-galactopyranoside -
Description
Red fluorogenic substrate for lacZ ß-galactosidase activity, intracellularly, or in vitro. -
Alternative names
- 3-Phenoxazone 7-(ß-D-galactopyranoside)
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Biological description
Resorufin ß-D-galactopyranoside requires only a single-step hydrolysis reaction to attain full fluorescence making it especially useful for sensitive enzyme measurements in applications such as ELISAs or high-content screening systems. Suitable for continuous measurement of enzyme activity at Ex/Em=573/585 nm. Can quantitate beta-galactosidaseactivity in single yeast cells via flow cytometry.
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Purity
98% -
CAS Number
95079-19-9 -
Chemical structure
Properties
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Chemical name
7-(β-D-Galactopyranosyloxy)-3H-phenoxazin-3-one
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Molecular weight
375.33 -
Molecular formula
C18H17NO8 -
PubChem identifier
125273 -
Storage instructions
Shipped at Room Temperature. Store at -20°C. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 50 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=CC2=C(C=C1O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)OC4=CC(=O)C=CC4=N2 -
Source
Synthetic