Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant vaccinia virus B18R protein (ab190383)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Tags: His-T7 tag N-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant vaccinia virus B18R protein
    See all B18R proteins and peptides

  • Biological activity

    Measured by neutralization of IFN-alpha effects on MCF7 cells in RT-PCR assay using IFN-alpha specific induced target gene index.

     

  • Purity

    > 95 % SDS-PAGE.
    The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P25213

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Vaccinia virus

    • Sequence

      MASMTGGQQMGRGHHHHHHGNLYFQGGEFELHSYAIDIENEITEFFNKMR DTLPAKDSKWLNPACMFGGTMNDIAALGEPFSAKCPPIEDSLLSHRYKDY VVKWERLEKNRRRQVSNKRVKHGDLWIANYTSKFSNRRYLCTVTTKNGDC VQGIVRSHIRKPPSCIPKTYELGTHDKYGIDLYCGILYAKHYNNITWYKD NKEINIDDIKYSQTGKELIIHNPELEDSGRYDCYVHYDDVRIKNDIVVSR CKILTVIPSQDHRFKLILDPKINVTIGEPANITCTAVSTSLLIDDVLIEW ENPSGWLIGFDFDVYSVLTSRGGITEATLYFENVTEEYIGNTYKCRGHNY YFEKTLTTTVVLE

    • Predicted molecular weight

      22 kDa including tags

    • Amino acids

      20 to 351

    • Tags

      His-T7 tag N-Terminus

    • Additional sequence information

      B18R cDNA was constructed with 31 N terminal T7/His tag from Vaccinia Virus.

    • Specifications

    Our Abpromise guarantee covers the use of ab190383 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituent: 0.32% Tris HCl

      Contains NaCl, EDTA, KCl, arginine, DTT and glycerol.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab190383? Please let us know so that we can cite the reference in this datasheet.

ab190383 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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