Recombinant vaccinia virus B18R protein (ab190383)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant vaccinia virus B18R protein
See all B18R proteins and peptides -
Biological activity
Measured by neutralization of IFN-alpha effects on MCF7 cells in RT-PCR assay using IFN-alpha specific induced target gene index.
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Purity
> 95 % SDS-PAGE.
The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Vaccinia virus -
Sequence
MASMTGGQQMGRGHHHHHHGNLYFQGGEFELHSYAIDIENEITEFFNKMR DTLPAKDSKWLNPACMFGGTMNDIAALGEPFSAKCPPIEDSLLSHRYKDY VVKWERLEKNRRRQVSNKRVKHGDLWIANYTSKFSNRRYLCTVTTKNGDC VQGIVRSHIRKPPSCIPKTYELGTHDKYGIDLYCGILYAKHYNNITWYKD NKEINIDDIKYSQTGKELIIHNPELEDSGRYDCYVHYDDVRIKNDIVVSR CKILTVIPSQDHRFKLILDPKINVTIGEPANITCTAVSTSLLIDDVLIEW ENPSGWLIGFDFDVYSVLTSRGGITEATLYFENVTEEYIGNTYKCRGHNY YFEKTLTTTVVLE -
Predicted molecular weight
22 kDa including tags -
Amino acids
20 to 351 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
B18R cDNA was constructed with 31 N terminal T7/His tag from Vaccinia Virus.
Specifications
Our Abpromise guarantee covers the use of ab190383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, EDTA, KCl, arginine, DTT and glycerol.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab190383 has not yet been referenced specifically in any publications.
