Recombinant rat ITK/EMT protein (ab196125)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 58% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant rat ITK/EMT protein
See all ITK/EMT proteins and peptides -
Biological activity
Specific Activity: 1.0 pmol/min/μg
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Purity
>= 58 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
PVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQE GAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSD YLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQV IKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFG VLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASPHVYQVMNHCW KEKPEDRPPFSRLLSQLAE -
Predicted molecular weight
57 kDa including tags -
Amino acids
352 to 620 -
Tags
GST tag N-Terminus
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Preparation and Storage
- EMT
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
Images
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SDS-PAGE - Recombinant rat ITK/EMT protein (ab196125)
10% SDS-PAGE analysis of ab196125 with Coomassie staining.
Lane 1: 20 µg Active rat ITK/EMT protein fragment (ab196125)
Lane 2: Protein marker -
Functional Studies - Recombinant rat ITK/EMT protein (ab196125)Enzyme reaction was conducted at 30°C for 1 hour with 2 μM Tyr peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted development reagent was added. Percentage phosphorylation was calculated based on the emission ratio of 445/520 nm after excitation.
