Recombinant mouse IL-12 protein (Active) (ab259419)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Cell Culture, Functional Studies, MS, HPLC, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with Phosphate Buffered saline. Reconstituted protein stable at -80C for 12 months or 4C for 1 week.
Images
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Functional Studies - Recombinant mouse IL-12 protein (Active) (ab259419)
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant Proliferation of CTLL-2 cells is 1.96 ng/mL corresponding to a Specific Activity of 5.10 x 105 IU/mg.
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SDS-PAGE - Recombinant Mouse IL-12 protein (Active) (ab259419)SDS-PAGE analysis of ab259419.
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HPLC - Recombinant Mouse IL-12 protein (Active) (ab259419)Purity: 100% (heterodimer)
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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Mass Spectrometry - Recombinant Mouse IL-12 protein (Active) (ab259419)M + 0. 9 Da (calc mass 21762.1) and M + 1.6 Da (calc mass 35848.4); heterodimer
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
