Recombinant human IL-12 protein (ab89013)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human IL-12 protein
See all IL-12 proteins and peptides -
Biological activity
Activity was measured by its ability to stimulate the proliferation of PHA-activated human T-lymphoblasts, and is typically in the range of 0.05-0.20 ng/ml. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical Sequence Alpha Chain: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPC TSEEIDHE DITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLA SRKTSFMMAL CLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNML AVIDELMQALNF NSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVT IDRVMSYLNAS Beta Chain: IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQ SSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIW STDILKDQKE PKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSR GSSDPQGVTCGA ATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPI EVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSW EYPDTWSTPHSYFSLT FCVQVQGKSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEW ASVPCS
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
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SDS-PAGE - Recombinant human IL-12 protein (ab89013)1D SDS-PAGE of ab89013 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab89013
Lane 2: ab89013 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab89013 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Appearance of additional band at lower MWt after treatment with PNGase F indicates the presence of N-linked glycans. The subsequent drop in MWt after treatment with a glycosidase cocktail indicates the presence of O-linked glycans. Additional high MWt bands in lane 3 are glycosidase enzymes. -
SDS-PAGE - Recombinant human IL-12 protein (ab89013)A sample of ab89013 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg of protein was loaded. Spot trains indicate presence of multiple isoforms. -
Functional Studies - Recombinant human IL-12 protein (ab89013)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangles indicate theoretical pI and MWt of the alpha and beta chains of the protein.
