Recombinant mouse AMPK alpha 2 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268340)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse AMPK alpha 2 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) -
Biological activity
The specific activity of ab268340 was 210 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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Purity
> 85 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Mouse -
Sequence
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQ KIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGE LFDYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAQ MNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSC GVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPDYLNRSVATLLMHMLQ VDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKF ECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGS FMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKA KWHLGIRSQSKACDIMAEVYRAMKQLGFEWKVVNAYHLRVRRKNPVTGNY VKMSLQLYLVDSRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRARS SFDSSTAENHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITAL AR -
Molecular weight information
SDS-PAGE molecular weight: ~68-72 kDa -
Amino acids
1 to 552 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_178143
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Amino Acid Sequence 2
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Species
Mouse -
Sequence
MGNTTSERVSGERHGAKAARAEGGGHGPGKEHKIMVGSTDDPSVFSLPDS KLPGDKEFVPWQQDLDDSVKPAQQARPTVIRWSEGGKEVFISGSFNNWST KIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINN LIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYVFRSEER FKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVM VLSATHRYKKKYVTTLLYKPI -
Molecular weight information
SDS-PAGE molecular weight: ~35 kDa -
Amino acids
1 to 271 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_182997
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Amino Acid Sequence 3
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Species
Mouse -
Sequence
MESVAAESSPALENEHFQETPESNNSVYTSFMKSHRCYDLIPTSSKLVVF DTSLQVKKAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKS ALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIH RLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLQELQIGTY ANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAA EKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLV VVDEHDVVKGIVSLSDILQALVLTGGEKKP -
Molecular weight information
SDS-PAGE molecular weight: ~40 kDa -
Amino acids
1 to 330 -
Tags
His tag N-Terminus -
Additional sequence information
GeneBank: NM_016781
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Preparation and Storage
Images
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Functional Studies - Recombinant mouse AMPK alpha 2 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268340)
The specific activity of ab268340 was 210 nmol/min/min in a peptide kinase assay using SAMStide (HMRSAMSGLHLVKRR) as substrate.
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SDS-PAGE - Recombinant mouse AMPK alpha 2 + AMPK gamma 1 + AMPK beta 2/PRKAB2 protein (Active) (ab268340)SDS-PAGE analysis of ab268340.
