Recombinant Human TrkA protein (Fc Chimera) (ab83562)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human TrkA protein (Fc Chimera)
See all TrkA proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical sequence: AAPCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTEL YIENQQH LQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSR LNLSF NALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGL GGV PEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRC Q VEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKN VTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQ P APSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHV NNG NYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDT NSTSE IPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRVV SVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISKAK GQPREPQVYTL PPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQ PENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL HNHYTQKSLSLSPGK -
Amino acids
33 to 405 -
Additional sequence information
Fusion of aa 1-405 of human TrkA and aa 93-330 of Fc region of human IgG1 (P01857). The chimeric protein was expressed in modified human 293 cells.
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Preparation and Storage
- gp140trk
- High affinity nerve growth factor receptor
- High affinity nerve growth factor receptor precursor
Images
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SDS-PAGE - Recombinant Human TrkA protein (Fc Chimera) (ab83562)Lane 1 – MW markers; Lane 2 – ab83562; Lane 3 – ab83562 treated with PNGase F to remove potential N-linked glycans; 10 μg protein loaded per lane; Deep Purple™ stained.
The drop in MW after treatment with PNGase F indicates presence of N-linked glycans. -
Functional Studies - Recombinant Human TrkA protein (Fc Chimera) (ab83562)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83562 exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.
The triangle indicates theoretical pI and MW of the protein.
