Recombinant human TPH2 protein (ab206456)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human TPH2 protein
See all TPH2 proteins and peptides -
Biological activity
Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH2. Fluorescence signal was measured at Em360nm/Ex300nm.
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMG YKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYC GYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQ YIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDED VQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAF DPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPY TQSIEILKDTRSIENV -
Predicted molecular weight
37 kDa including tags -
Amino acids
151 to 466 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.09% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human TPH2 protein (ab206456)
Activity assay using ab206456
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SDS-PAGE - Recombinant human TPH2 protein (ab206456)6-20% SDS-PAGE stained with Coomassie Blue.
Lane 1: ab206456 (5 µg)
Lane 2: Protein Marker