Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TPH2 antibody [EPR19191] (ab184505)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19191] to TPH2
Suitable for: WB, IHC-P, IP, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-TPH2 antibody [EPR19191]
See all TPH2 primary antibodies
Description

Rabbit monoclonal [EPR19191] to TPH2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse midbrain and brainstem lysates. IHC-P: Mouse and rat brainstem tissues. IHC-Fr: Mouse brainstem and cerebrum tissue. IP: Mouse brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19191
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling TPH2 with ab184505 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of paraffin-embedded Mouse brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TPH2 antibody [EPR19191] (ab184505)

All lanes : Anti-TPH2 antibody [EPR19191] (ab184505) at 1/1000 dilution

Lane 1 : Mouse midbrain lysate
Lane 2 : Mouse brainstem lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

This target is specifically expressed in DR (dorsal raphe nucleus). WB was tested with midbrain or brainstem because both include the DR zone. The results were consistent with the literature (PMID:21272616; PMID: 19181488).
Immunoprecipitation - Anti-TPH2 antibody [EPR19191] (ab184505)

TPH2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab184505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184505 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse brain lysate, 10µg (Input).

Lane 2: ab184505 IP in Mouse brain lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184505 in Mouse brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Immunohistochemistry (Frozen sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brainstem tissue labeling TPH2 with ab184505 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of paraffin-embedded Rat brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of rat brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse spleen. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPH2 antibody [EPR19191] (ab184505)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-TPH2 antibody [EPR19191] (ab184505)

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