Recombinant human TIMP1 protein (ab82104)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Preparation and Storage
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Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week). For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
Images
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Sandwich ELISA - Recombinant human TIMP1 protein (ab82104)Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
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Western blot - Recombinant human TIMP1 protein (ab82104)
